![]() ![]() Now all of a sudden it Only shows up in a totally different state and with exact name search. Is there something I could do to send signals to Google to show that I am in Matthews, NC?Ģ months ago my listing quit showing up at all unless you typed exact business name What could possibly cause my listing or Google to do this? I have been without my listing for a few months now and have NO calls coming in from it. If you search Locksmith Independence, KS it shows up on the maps. If you search Locksmith Matthews, NC my listing does not show up at all. Keep in mind the GMB is in Matthews, NC All my service areas and the actual map show the correct areas. Now if I search my business name under the auto populate I see it with Independence, KS on the listing. I pretty much do not have any traffic, views or calls now. Posted about my SAB listing a few weeks ago about not showing up in search only when you entered the exact name. The downstream EF-1 alpha core promoter starts the expression of GFP. PiggyBac Dual promoter (PB513B-1) is a plasmid for mammalian cell transposition experiment, located at the downstream of CMV promoter, MCS, so that the target gene or microRNA is more convenient to clone. "Efficient selection for high-expression transfectants with a novel eukaryotic vector". Basic analysis for a user-entered sequence includes restriction sites and map. PiggyBac Dual promoter (PB513B-1) Description. ^ Niwa H, Yamamura K, Miyazaki J (December 1991)."Expression vector system based on the chicken beta-actin promoter directs efficient production of interleukin-5". ^ a b Miyazaki J, Takaki S, Araki K, Tashiro F, Tominaga A, Takatsu K, Yamamura K (July 1989)."The CMV early enhancer/chicken beta actin (CAG) promoter can be used to drive transgene expression during the differentiation of murine embryonic stem cells into vascular progenitors". ^ Alexopoulou AN, Couchman JR, Whiteford JR (January 2008)." 'Green mice' as a source of ubiquitous green cells". ^ Okabe M, Ikawa M, Kominami K, Nakanishi T, Nishimune Y (May 1997).NEB to find out how many additional flanking nucleotides to add at the 5. ![]() As well as increases the expression of transgenes under low temperature conditions. Include 1 (for staggered/blunt end) or 2 terminal restriction sites. Some of the CAG promoter's functions as an effective tool that increases and maintains the expression level of recombinant proteins. The 3' part of the promoter has high GC content and is thus refractory to PCR amplification. In addition to the CMV immediate early enhancer, the intron of the chicken beta actin gene contains an enhancer element, which is highly conserved among vertebrates. Īlthough the whole construct is commonly referred to as the "CAG promoter", it is not a promoter in a strict sense, as it includes a part of the transcribed sequence (the first exon and the first intron of chicken beta-actin gene) and enhancer elements. The initiation codon located at the proximal region of the second exon was disrupted by digesting with NcoI restriction enzyme and replacing the site with a HindIII linker. The resulting synthetic element was used in the pCAGGS expression vector. ( C) the cytomegalovirus (CMV) early enhancer element, ( A) the promoter, the first exon and the first intron of chicken beta-actin gene, ( G) the splice acceptor of the rabbit beta-globin gene ĬAG promoter was constructed in the lab of Dr Jun-ichi Miyazaki from the following sequences: The CAG promoter is a strong synthetic promoter frequently used to drive high levels of gene expression in mammalian expression vectors. ![]()
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